mouse anti pgk1 antibody  (Novus Biologicals)

Journal: eLife

Article Title: Glycolysis-dependent sulfur metabolism orchestrates morphological plasticity and virulence in fungi

doi: 10.7554/eLife.109075

Figure Lengend Snippet: ( A ) Epitope-tagged strains of various proteins involved in the de novo biosynthesis of sulfur-containing amino acids, including Met4, Met32, Met16, Met10, Cys4, and Cys3 were spotted on SLAD in the presence and absence of sub-inhibitory concentration of 2-Deoxy-D-Glucose (2DG) following which, cells from colonies were isolated at day 5 and levels of these proteins were assessed using Western blotting. Raw images of Western blots were analyzed using ImageJ to normalize targeted protein expression with the expression of housekeeping protein- β-actin, in order to generate densitometric graphs. Statistical analysis was done using unpaired t-test, **(p<0.01) and *(p<0.05). Error bars represent SEM. ( B ) Epitope-tagged strains of various proteins involved in the de novo biosynthesis of sulfur-containing amino acids, including Met32, Met16, Met10, and Cys3 were grown in liquid SLAD and then treated with sub-inhibitory concentration of 2DG for 24 hr after which protein expression was checked using Western blotting. Pgk1 was used as loading control. ( C ) Raw images of Western blots were analyzed using ImageJ to normalize targeted protein expression with the expression of housekeeping protein-Pgk1, in order to generate densitometric graphs. Statistical analysis was done using unpaired t-test, ***(p<0.001), **(p<0.01) and *(p<0.05). Error bars represent SEM. This figure was created using Biorender.com. Figure 2—figure supplement 2—source data 1. Uncropped and labeled blots for . Figure 2—figure supplement 2—source data 2. Raw unedited blots for .

Article Snippet: Pgk1 Mouse mAb (Cat. #sc-130335) was purchased from Santa Cruz Biotechnology.

Techniques: Concentration Assay, Isolation, Western Blot, Expressing, Control, Labeling